The former Porto player, who is yet to make his debut for the Italians after suffering a calf injury, was expected to be ready to return around February 24, but has been left out as a precaution as the new Argentine coach prepares for February 29's friendly in Miami.
Aside from Guarin, Pekerman takes a strong-looking squad to the United States. Racing Club playmaker Gio Moreno returns to the fold after being overlooked by former trainer Leonel Alvarez, and rejoins club team-mate Teofilo Gutierrez in the 22-man squad.
There is also a place for Atletico Madrid's Radamel Falcao, who had missed out on the Cafetero's qualifying clash Argentina due to injury.
Full Colombia Squad:
|Luis Martinez||Once Caldas
|Dayro Moreno||Once Caldas