The Italy international has warned his team-mates not to underestimate the Viola as they will prove to be a difficult opponent
The centre-back believes the Viola will be Juve's toughest opponent up till date, and has stressed that the reigning champions will have to be on top of their game if they want to continue their 43-game unbeaten run.
"In my opinion, Tuesday's game will be the most difficult match of the season so far," Bonucci was quoted as saying on the club's official website.
"The match in Florence will be difficult for a thousand and one reasons. They lost 5-0 against us last term, so it will be the game of the season for them. A difficult challenge awaits us.
"We are completely focused on Fiorentina. We will have to show them respect of we could be in trouble on Tuesday."
The match at the Artemio Franchi is scheduled to kick off at 20:45CET.