The Rossoneri chief says the Eastlands club are chasing the former Barcelona coach, in a move which is set to cast yet more doubt over the future of Roberto Mancini
The 41-year-old left the Catalans at the end of last season to take a break from football yet that has not prevented his future from being the subject of intense speculation.
Guardiola has been heavily linked with a move to Chelsea, despite the recent arrival of Rafael Benitez, and Berlusconi claims there are several Premier League teams chasing the former Barcelona coach.
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"There are some English clubs after Guardiola, especially [Manchester] City who have directors that we know very well," Berlusconi told reporters.
“We will try to sign him, but it’s going to be tough.”
The former Italian prime minister also dismissed reports suggesting he was interested in signing Mario Balotelli from Manchester City adding: "A swap deal including [Alexandre] Pato and [Mario] Balotelli? I don't like making exchanges."