Steven N'Zonzi had a huge opportunity to be the hero for Sevilla when he stepped up to the penalty spot in the closing minutes against Leicester City.
But the midfielder choked under pressure in the Champions League last-16 second leg, sending a weak shot right into the chest of diving goalkeeper Kasper Schmeichel.
The save sealed a 2-0 victory for the Foxes, who move on to the quarter-finals with a 3-2 aggregate win over the Spanish side.
Schmeichel was called into action on the penalty after picking up a yellow card for running into Sevilla's Vitolo in the box.
Schmeichel has saved both of his penalties in the Champions League this season, with both coming in each leg against Sevilla.
Sevilla are also the first team to miss a penalty in each leg of a Champions League knock-out round since Bayern Munich in 2013-14 against Arsenal.