The Barcelona star picked up a muscular injury this weekend and is one of a number of withdrawals for the clash with Germany, with Nico Gaitan also drafted in
The Barcelona forward, who starred for the Albiceleste this summer in Brazil, featured throughout Barcelona's 1-0 win at Villarreal on Sunday but the club confirmed afterwards that he had picked up a knock at El Madrigal.
Barca's report to the AFA claimed: "The player suffered a muscle strain in his right hamstring and needs rest as a precaution."
Lamela failed to earn a place in Argentina's World Cup squad after a debut season to forget a Spurs, but joins Nico Gaitan as late additions to the squad for Wednesday clash.
Messi is not the only late withdrawal from Gerardo Martino's first match as Argentina boss, who was named as Alejandro Sabella's successor last month, in their clash with newly crowned world champions Germany in Dusseldorf.
Albiceleste veteran Maxi Rodriguez suffered tightness in his hamstring, Inter striker Rodrigo Palacio's left ankle is bruised and Zenit defender Ezequiel Garay's left leg has been causing him muscular pain ahead of the international break.
Paris Saint-Germain attacker Ezequiel Lavezzi is still to be evaluated by Argentina's medical team after complaining of backache following his club's 5-0 victory over Saint-Etienne on Sunday.