The 20-year-old injured his ankle during Monday night's 3-1 win over Wales Under-21s while playing for England Under-21s, ruling him out of the Under-20s competitionHarry Kane will miss England Under-20s' Toulon Tournament after suffering an ankle injury in the Under-21s win over Wales on Monday.
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Manager Gareth Southgate had planned to take Kane to the Toulon Tournament, which starts later this month, so the former Middlesbrough boss was left to rue the 20-year-old's absence.
"It's a real shame because Harry has been extremely patient for his chance and I was looking forward giving him some of the prestigious games in Toulon," he told the Football Association's official website.
"He's warranted that opportunity and has had a super finish to the season with Tottenham so it's disappointing for us and for him."
Kane scored three goals in six Premier League starts for the London club this season.