The Azzurri boss has all but ruled out taking the 37-year-old to Brazil despite rumours that he was considering attempting to lure the forward out of international retirement
The Roma captain has not featured for his country since helping the Azzurri lift the trophy in Germany in 2006, but there has been recent mounting speculation that the 37-year-old forward may be prepared to come out of international retirement should he be called upon in the summer.
However, Prandelli has now all but ruled out the possibility of Totti travelling to Brazil with Italy.
"He is a great talent, but time does not stand still for anyone," the Italy boss told reporters.
"Either way, let's not talk about a Totti return. Last time we did that, it didn't end well for him as he picked up an injury.
"And let's not forget that he was the one who made the decision to retire from international football."
Totti, 37, has made 58 international appearances, scoring nine goals in the process. His Roma side currently sit atop Serie A, yet to lose a league game this season with 12 fixtures played.