The Barcelona star played the last half hour of Argentina's World Cup qualifier against Colombia but is still not fully fit
The Argentina international featured for just over half an hour of the Conmebol game against Colombia, but warned afterwards he may be incapable of playing what would be his second game in a week.
"I felt good, but what with [having played] little football and not knowing how the [injured] leg was going to respond, I don't want to take chances," Messi told a post match press conference.
The Barcelona forward also expressed concern that his problems could be compounded by having to play at a great altitude, something that is unavoidable given the location of Ecuador's stadium.
He added: "To play at a high altitude is really tiring, it will be a hard game and I come without many minutes."
Messi also addressed the game against Colombia, which finished 0-0, arguing that despite having "many chances to score", Argentina were "somewhat handicapped" by refereeing decisions going against them.
Argentina lead the Conmebol table five points ahead of second-placed Colombia.