The Azzurri boss revealed on the eve of the game in Genoa that he would be fielding Paris Saint-Germain midfielder Motta in an unfamiliar playmaking role in behind two strikers with a combined total of ten Azzurri caps.
Elsehwere, Juventus duo Andrea Pirlo and Claudio Marchisio will team up with AC Milan’s Antonio Nocerino in the midfield.
Andrea Barzagli and Angelo Ogbonna have been paired together at the heart of the Azzurri defence and will have Christian Maggio and Domenico Criscito outside them.
Veteran Juventus goalkeeper Gianluigi Buffon continues in nets.
As for USA, Everton’s Tim Howard returns in between the sticks, while Steven Cherundolo and Carlos Bocanegra, who will skipper the side, are recalled to the defence.
Maurice Edu and Michael Bradley will anchor the midfield, while Fulham's Clint Dempsey features in an attacking triumvirate tucked in behind lone striker Jozy Altidore.
Italy (4-3-1-2): Buffon; Maggio, Barzagli, Ogbonna, Criscito; Marchisio, Pirlo, Nocerino; Thiago Motta; Matri, Giovinco.
USA (4-2-3-1): Howard; Cherundolo, Goodson, Johnson, Bocanegra; Bradley, Edu; Williams, Dempsey, Shea; Altidore.