The centre-back is set to sit out Wednesday's vital Primera Division clash with Sevilla after the capital club failed to have his dismissal against Barcelona quashed
The 27-year-old centre-back, who has been handed a one-match ban, was sent off for the 19th time in his career when he was adjudged to have felled Neymar as the Barcelona forward bore down on goal in Los Blancos' 4-3 Clasico defeat at the Santiago Bernabeu.
The capital club had hoped to have the Spain international's suspension quashed to allow him to participate in Wednesday evening's match at Sevilla but the RFEF has decreed otherwise.
However, Madrid have indicated that they will make a last-ditch attempt to have the World Cup winner available for the trip to the Estadio Ramon Sanchez Pizjuan, as they will lodge a case with the RFEF's Appeals Court and could even go as far as the Spanish Sport Ministry's Administrative Court, which would mean that the defender's ban would be postponed.
Madrid, who are now second in La Liga behind Atletico Madrid on a head-to-head basis, have included Ramos in their squad for their must-win clash with in-form Sevilla.