The 26-year-old centre-back is struggling once again with his recurring groin problem, meaning that he could be left behind for his side's Primera Division trip to Seville
The Spain international has been struggling with a groin problem this season and, while he played all 90 minutes of Wednesday's Champions League win over AC Milan, the 26-year-old could be rested for the trip to Seville.
A statement on the club's website read: "Pique underwent tests [on Thursday] on his groin problem.
"Developments over the next few days will determine whether he is fit enough to be considered for selection against Betis at the weekend."
Barcelona currently lead La Liga from Atletico Madrid, having dropped just two points over the course of the opening 11 rounds of the season.