The stopper was unable to participate in the Blaugrana's last training session before Sunday's crunch derby, leaving Mascherano and Song as the most likely centre-backs
Barcelona have confirmed that Gerard Pique will miss the Clasico against Real Madrid after failing to recover from an ankle injury in time.
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But despite also participating in a work-out with the rest of his team-mates on Saturday, Pique failed to train in Sunday's last session, instead spending much of midday undertaking specific gym work.
The call has since been made by coach Tito Vilanova to not include the former Manchester United man in his squad following a meeting between the duo, and it looks increasingly likely that Javier Mascherano and Alex Song will deputise in defence.
However, young trio Cristian Tello, Sergi Roberto and Oier Olazabal were present and could yet be named in the former assistant's final party for the visit of Madrid.