Jose Mourinho shuffles his pack ahead of the Champions League semi-final first leg away to Bayern Munich on Tuesday night
Madrid need a win to maintain their four-point lead over Barcelona in La Liga, a week ahead of the Clasico clash at Camp Nou.
But Mourinho appears confident of beating Sporting, despite losing the corresponding fixture last season.
Callejon comes in as Kaka and Angel Di Maria are left out, while Higuain starts ahead of Karim Benzema up front, and Marcelo returns at left-back in place of Fabio Coentrao.
Nuri Sahin replaces the suspended Xabi Alonso.
Real Madrid (4-2-3-1): Casillas; Arbeloa, Sergio Ramos, Pepe, Marcelo; Sahin, Khedira; Ozil Callejon, Cristiano; Higuain
Subs: Adan, Kaka, Benzema, Granero, Coentrao, Albiol, Di Maria
Sporting Gijon (4-5-1): Juan Pablo; Galvez, Botia, Hernandez, Canella; Moises, Lora; Damian Suarez, Trejo, De las Cuevas; Sangoy
Subs: Cuellar, Lora, Nacho Novo, Eguren, Suarez, Ayoze, Colunga