The 30-year-old, who has netted against both Copenhagen and Galatasaray in the tournament so far, has been ruled out for a minimum of three weeks as he recovers from an adductor injury, meaning that he will play no part in the upcoming fixtures with the Liga giants.
Consequently, the Italy international will also sit out domestic clashes with Fiorentina, Genoa, Catania and Parma respectively, but could return for the visit of former club Napoli on November 10.
"Fabio Quagliarella picked up an injury yesterday, and tests which he underwent this morning have revealed damage to his right adductor longus muscle," a statement on the club's official website read.
"He is expected to be out of action for 20 days."Antonio Conte's men face the Blancos in back-to-back group games knowing that two defeats would significantly dent his team's hopes of progressing to the latter stages of the competition, with the Bianconeri yet to pick up a victory in Group B.
The reigning Italian champions travel to the Santiago Bernabeu for the first clash with Carlo Ancelotti's men on October 23, before welcoming them to Turin two weeks later.