The Montenegro star is being plagued by a thigh problem and will be unavailable for a while due to the knock
The attacker picked up the knock in his side's 2-0 Serie A win over Cesena last weekend and was initially expected to be match fit in time for Thursday's cup game.
However, the Turin side have now confirmed on their official website that Vucinic won't play against Bologna due to the knock.
It's not yet known exactly how long the Montenegro international will be out of action for due to the thigh problem, but Juve fear that the 28-year-old could be out of action until Christmas.
Vucinic joined Juventus from Roma in the summer transfer window and has since developed into an important first team member for the Bianconeri. The striker has so far scored twice in 12 Serie A appearances for the Old Lady.
The Coppa Italia game in Juventus Stadium kicks off at 20.00 GMT.
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