The Brazilian ace hails the supporters as the club's 12th man while centre-back Nesta is confident the side can continue their Scudetto charge, even without the banned Ibrahimovic
The San Siro outfit are three points above Napoli in the table, and have a great chance to extend their lead with Samp winless in their last eight games.
Napoli, on the other hand, have a tough clash against Udinese who are chasing a Champions League place, offering Milan a chance to take advantage.
Pato, who has struck 14 goals in 21 league games this term, has called on the Milan fans to get behind the team and help them achieve domestic glory.
"It's a difficult game for us. I want it to be like the derby, with 80,000 fans shouting and supporting us because it's important that the fans help us as they are the 12th man on the pitch," the Brazilian told Milan Channel.
"I am sure that with their support we will win this championship."
Centre-back Alessandro Nesta, meanwhile, has backed his side to continue their title charge, even without Zlatan Ibrahimovic who is currently serving a three-game ban.
"We all know Ibrahimovic is important, for us he is fundamental, but we won the derby without him. If he plays it's better for us because he gives an added value," Nesta told Sky Sport Italia.
"However, we have players like Robinho, [Alexandre] Pato, [Clarence] Seedorf, and so we move forward as Milan have a group of players which can go on regardless of everyone else."
And with Napoli just three points behind, the former Italy international admits they are starting to feel the bite.
"There is pressure for us, for them, for everyone. It's the same for all," he added.