The Juventus veteran left training with an ankle problem ahead of the Group D opener against Roy Hodgson's side, who have their own concerns surrounding left-back Luke Shaw
Italy goalkeeper Gianluigi Buffon was forced out of training with an ankle injury on the eve of his country's World Cup opener with England in Manaus.
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Azzurri coach Cesare Prandelli confirmed the injury to the Juventus keeper, but played down its significance.
"How do you know this, you were not supposed to have seen us," he replied when asked by an Italian reporter why Buffon limped from the pitch.
"It is a very, very light ankle twist. He is receiving treatment at the moment but it is nothing serious."
England left-back Luke Shaw also appeared to have picked up a knock during his country's light session at the Amazon stadium.
The Southampton youngster, who has agreed a record-breaking deal to join Manchester United, walked gingerly down the tunnel and indicated an impact injury to medical staff.
Unlike Italy, the Three Lions' training session took place after their press conference, so the extent of the problem could not be addressed by manager Roy Hogdson.