The Spireites can extend their lead at the top of the table with victory over third-placed Dons, but Oxford United can keep up the pressure with a win over Cheltenham Town
Leaders Chesterfield can extend their unbeaten run in League Two this season to seven games when they host AFC Wimbledon on Saturday.
Paul Cook's men moved to the top of the standings with a 1-0 win over bottom side Accrington Stanley thanks to Tendayi Darikwa's second half-goal last time out.
This victory represented Chesterfield's second consecutive clean sheet and defender Richie Humphreys believes his side's good start to the season has been built on a resolute defence.
"I thought we were brave, held a good line, enabled (goalkeeper) Tommy (Lee) to have that space to come and catch the ball and relieve the pressure," he told the club's official website.
"If you keep a clean sheet you’ve got every chance of winning a football game."
However, Wimbledon go into the fixture at the Proact Stadium high on confidence after moving up to third in the league with a 2-0 win over York City in their previous game.
Second-placed Oxford United will hope Wimbledon can take points off the pacesetters as they travel to Cheltenham Town.
Chesterfield and Oxford are the only sides yet to lose a game this season, while Cheltenham have not won in their last two.
Meanwhile, the division's bottom two sides, Hartlepool United and Accrington, meet at Victoria Park on Saturday with both in danger of being cut adrift at the foot of the table.
Hartlepool lost again last time out as Wycombe Wanderers beat them 2-1 at home, but Matty Dolan believes his team-mates can turn their form around.
"I think once we get that first win in the league, the confidence will come through for us to on a run," he told the club's official website.
"I’ve been in environments where the team has gone on runs of eight or nine wins and that’s what the feeling is like here; it does feel like it is coming, we just have to keep doing the things that gaffer is telling us."
League Two's second-highest goalscorers Fleetwood Town are able to move into the play-off zone when they host Bury, while Morecambe can make it four wins in a row against Newport County.
Northampton Town and York City both sit two points clear of the relegation zone and host Exeter City and Mansfield Town respectively.
Burton Albion can secure their first win in four games against Portsmouth, while Southend United could move into the play-off spots with victory over Scunthorpe at Roots Hall on Friday.
Elsewhere, Rochdale, fresh from their Greater Manchester derby win against Bury last Saturday, welcome Torquay United.
But Rochdale manager John Coleman will be without captain Peter Cavanagh for the clash, after the midfielder was ruled out up to six weeks with an Achilles problem.
Just a point separates Bristol Rovers and Dagenham and Redbridge before the sides meet at Victoria Road on Saturday, while Wycombe Wanderers can make it four games unbeaten against Plymouth Argyle.