Despite losing Uwe Rosler to Wigan, new Bees manager Mark Warburton has kept momentum going and will be looking to continue their fine run at Deepdale on Saturday
Both sides are currently nine games unbeaten in the league and separated by two points in third and fourth place.
Brentford, who are above Preston, lost manager Uwe Rosler to Wigan earlier this month but new boss Mark Warburton got his reign underway with victory over Oldham Athletic last weekend.
Victory for either side could move them closer to Wolves, if Kenny Jackett's second-placed side fail to take any points at Rotherham United.
Wolves have lost their last two matches and face a tough task to collect three points at the New York Stadium with Rotherham unbeaten in six league games.
Leyton Orient have failed to take full advantage of Wolves' recent slip-ups, meaning the former hold a slender, one-point advantage at the top of the table.
Orient host John Gregory's Crawley Town this weekend, with the ex-Aston Villa boss searching for his maiden win and Crawley's first in the league since mid-October.
Two sides in play-off contention - Peterborough United and Bradford City - meet at London Road.
Graham Kavanagh's Carlisle United will aim for their third win in a row when they play Walsall, Port Vale face Milton Keynes Dons and the County Ground will see Swindon Town entertain Coventry City.
At the other end of the table, two clubs with recently appointed managers will do battle at Meadow Lane as Sean Derry's Notts County host Steve Cotterill's rock-bottom Bristol City.
County have won their last two matches - scoring seven goals in the process - while City are yet to benefit from a new-manager bounce and have not won in five league outings.
Stevenage face a home game against Sheffield United and would leapfrog their opponents with victory.
Elsewhere, struggling Crewe Alexandra face a home game against Shrewsbury Town, Tranmere Rovers host Gillingham and Oldham Athletic entertain Colchester United.