A Cristiano Ronaldo header in extra time secured the trophy for Jose Mouringo's men but the Barcelona coach believes his side need to maintain their focus ahead of ther title run-in.
"This game means that there is a winner and a loser, nothing more or nothing less," said Guardiola.
“We will have to pick ourselves up because there is not much time until the end of the year and we need to see how it ends.
“It is always better to win than lose, but we have to recover. We are in a good position in the league and we have to make sure we keep going.
“We have our next league game on Saturday and I hope people recognize how much we have left to fight for.”
Guardiola took the opportunity to thank the fans and their behavior despite the result.
“We have to digest this. They played a good first-half; we improved in the second but with a team like this [Real Madrid] this can happen," he continued.
“They have played directly, won the second balls and made it a very fast game. I think we played well. I know that sounds bad to say after losing but we had a good second half and played well in extra time, but it’s never easy when you are playing against such strong players.
“We must continue and not become disunited. We must win in the league first, and then try and get to Wembley [for the Champions League final]."