Tottenham travel to Rome for Thursday's Europa League match against Lazio and the Portuguese coach is hoping that the Italian fans do not repeat their White Hart Lane behaviour
When the two clubs faced each other at White Hart Lane in October, Jermain Defoe, Andros Townsend and Aaron Lennon were subject to abuse from the travelling band of away supporters, resulting in a £32,500 fine from Uefa.
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Speaking at his pre-match press conference, Villas-Boas told reporters: "Uefa decided to punish Lazio and rightly so. [But] It doesn't mean that incidents won't happen again.
"I think the players are in the right frame of mind to leave the authorities to deal with those situations in case it happens.
"That's something that hopefully will not happen tomorrow."