Real Madrid will be in pot two of the Champions League group stage draw for the first time since 2010-11 thanks to their failure to win the competition or La Liga this term, and will be joined by Arsenal and Manchester United.
Carlo Ancelotti's side failed to defend their European crown, with Juventus eliminating them 3-2 on aggregate at the semi-final stage in midweek, and have subsequently dropped down a pot, joining the Gunners and Louis van Gaal's side.
Both Premier League sides have confirmed their places in the top-four this season but, on current league positions, are set to finish below Chelsea and Manchester City, with Jose Mourinho's side listed in pot one, while United are set to face a play-off to qualify for the group stages.
In previous years, the pots were decided by each team's coefficient ranking but the governing body has ruled that winners of the top seven leagues and the European champions will now fill pot one.
As Juventus and Barcelona, the two Champions League finalists, have already won their domestic titles, one spot will be granted to the Eredivisie - the eighth-ranked league in 2013-14.
The drop means Madrid could end up facing the likes of Bayern Munich, Chelsea, Juventus or Paris Saint-Germain in the group stages next season.
Other pot one regulars who will have to make do with a place in pot two include Atletico Madrid and Porto.
The final for the 2015-16 Champions League takes place at San Siro in Milan on May 28.
Pot one (confirmed): Barcelona, Bayern Munich, Chelsea, Juventus, Paris Saint-Germain, Benfica, Zenit, PSV.
Pot two (on current league positions): Real Madrid, Atletico, Manchester United*, Porto, Arsenal, Valencia*, Bayer Leverkusen*, Man City (*must play qualifying round)