Diego Simeone's men held the Blaugrana to a 1-1 draw at Camp Nou in the reverse fixture last week and Raul Garcia says the Rojiblancos are poised to realise a "dream".
"Atletico will be ready for the game against Barcelona. We are high on confidence following the good results," the midfielder told Marca.
"We want to find right until the end and see how far we can go. We are all very pleased with what we have done so far. This season might seem like a dream, but it's actually reality.
"This is the result of a lot of hard work over the years. We have worked hard and are brimming with confidence.
"We all know how important this game is both for the club and for the fans. We are all determined to do well."
Atletico are still unbeaten against Barcelona this season, drawing all four encounters with the Catalans, and know that a 0-0 scoreline would be enough to send them through to the last four of the Champions League.