The 28-year-old netted three goals in last season's group stages, including a strike at the Camp Nou, and already has six goals in all competitions for the Scottish champions this term.
And fresh from netting a hat-trick at the weekend against Kilmarnock, Lennon has highlighted the importance of the Greece international playing well against the current La Liga leaders.
|14/1||Georgios Samaras is 14/1 with Paddy Power to open the scoring against Barcelona
"You all saw on Saturday what a talent he is and when he plays like that he is very difficult to stop.
"I hope that he hasn't peaked too early because I feel that to get something out of the game I need Samaras to have a big game.
"In the Nou Camp, before he went off injured, he was causing Barcelona lots of problems and I am hoping for more of the same tonight."
Lennon is also hoping Fraser Forster will play to his capabilities in goal, adding: "What you need against Barcelona is a fair amount of luck if you want things to go well way on the night and you need your goalkeeper to play very well.
"And in Fraser Forster I have got one of the best goalkeepers in the competition."