The club have announced their decision, and they will be taking legal action against the Real Madrid coach following the Portuguese tactician's controversial comments after defeat
"Yesterday Mr Mourinho went over all the limits. We trust in Uefa measures," said board member and club secretary Antoni Freixa Martin.
"The president decided to convene a special meeting and we will report the coach of Real Madrid to the Control and Disciplinary Committee of Uefa for his statements. They constitute a fraction of Article 5. Barcelona feels hurt.
"We cling to point B and D of the article because we feel the club's reputation has been damaged.
"We have reacted because there has been an offence against the club, players, staff and fans."
The Catalan side has taken action after Mourinho suggested Barcelona's successes were based on scandal.
Mourinho said: "Pep Guardiola is a fantastic football coach, but he won a Champions League following the scandal of Stamford Bridge. I would have been ashamed to win it like that. And this year, if he wins, it will be with the scandal of the Bernabeu," Mourinho said after the game.
It's this sentence which has aggreived the Camp Nou outfit who will thus present their case to Uefa.
Earlier on Thursday, Uefa said they had launched their own investigation into matters following the incidents during the game.