The England midfielder has not played for his club since April 4 thanks to a knee injury and had been hopeful of returning to face Manchester City on Sunday but is finally training again and ready to play.
"Aaron has trained with the team and is back in contention. It's good to have him back," Villas-Boas told reporters. "He's a player who has had a great impact this season."
The former Chelsea boss also reserved praise for Jermain Defoe, who ended a barren run of 12 games by scoring an eye-catching goal after coming off the bench against Roberto Mancini's men.
|10/1||Aaron Lennon is 10/1 with William Hill to score the first goal in Wigan v Tottenham
"Yes, it's a big decision [to choose between Defoe and Emmanuel Adebayor] but we are extremely happy," he explained.
"It's very important for us to have Jermain back, particularly because he found the back of the net. Goalkeepers have been making impossible saves against him and he finally got the ball in the net the other day with a tremendous goal that helped us get the three points.
"It's good because he had a blip in terms of goals, not performances - he was always sharp - but he had a couple of injuries. Now he is back he gives us that quality and confidence by scoring."
The Portuguese added that his side would have to stay motivated against the relegation-battling Latics, just the same as for their stunning 3-1 comeback win against City.
"It's a totally different proposition [to City] but just as important," he warned. "Wigan's ambitions are as important as Manchester City's and ours. We expect a difficult game.
"We have to understand that only the same motivation as playing against the champions will give us the conditions to win this game.
"All of our remaining five games are vital. Our destiny is in our hands and we will try to get as many points as possible."