The Saints boss urged his squad to keep looking forward after their loss to the bottom-of-the-table Rs on Saturday left them just three points clear of the relegation zone
After Gaston Ramirez levelled proceedings following an early Loic Remy opener, Jay Bothroyd restored the lead for the Rs with 10 minutes remaining and left Pochettino lamenting his side's defensive lapses.
And with next Saturday's clash with Norwich on his mind, Pochettino was clearly intent on focusing on games ahead, rather than dwelling on missed opportunities, despite his disappointment at the result.
"We can't look back on this result - we just have to prepare for Norwich, I have trust and confidence in my team. From now on, we just need to keep on working and going forward," he told reporters.
"We didn't expect this result, but we have to accept it because we made some mistakes. We tried very hard and put in a great effort to get a different kind of result but, because of the mistakes we made and their goalkeepers' interventions, we didn't get a positive one."
Southampton's problems were exacerbated by a first-half injury to captain Adam Lallana, though Pochettino was keen not to pin their poor performance on the loss of one influential figure.
"Lallana is a very important player for us, and to lose him at that stage of the game was very unfortunate for us." he continued.