The winger missed the 4-2 defeat to Chelsea last weekend but is restored to the starting XI to face his former club, who bring Rickie Lambert back into their team in attack
The winger missed the 4-2 defeat against Chelsea at White Hart Lane after his partner went into labour. Gylfi Sigurdsson drops to the bench in an otherwise unchanged XI.
Brad Friedel continues to be preferred to Hugo Lloris in goal, with the Frenchman again included among the substitutes. Emmanuel Adebayor's back injury has ruled him out of the squad entirely.
Southampton boss Nigel Adkins, meanwhile, makes two changes to the team that lost 4-1 against West Ham eight days ago.
Rickie Lambert is restored to the attack in place of Guly Do Prado, who drops to the bench, while Jos Hooiveld misses out as Danny Fox returns at left-back. Maya Yoshida will shift to central defence.
Southampton: Boruc; Clyne, Fonte, Yoshida, Fox; Schneiderlin, Steven Davis; Puncheon, Lallana, Rodriguez; Lambert.
Subs: Kelvin Davis, Hooiveld, Ward-Prowse, Guly, Mayuka, Chaplow, Reeves.
Tottenham: Friedel; Walker, Gallas, Caulker, Vertonghen; Sandro, Huddlestone; Lennon, Dempsey, Bale; Defoe.
Subs: Lloris, Naughton, Dawson, Livermore, Townsend, Falque, Sigurdsson.