The striker, who hit the winner with virtually the last kick of the game, came on in place of the former Newcastle midfielder after 60 minutes.
Barton claimed post-match that his performance was the “worst I've ever played in my career”, however Mackie still wasn't pleased to hear the negative reception from supporters.
"You don't like to see that, especially towards your captain,” Mackie told reporters.
"Joey is an immensely strong character so I don't think we need to worry about him.
"He was probably the happiest lad in the changing room after the match.
"He is the team captain and he wants us to win games whether he is banging the goals in or not and we are lucky to have him.
"He is our captain and he has probably got the biggest part to play in the run-in."
The win, just their second in 17 games, lofted Mark Hughes' men out of the drop zone, but with fixtures against the top five teams in the league in their last nine games of the season Mackie knows the battle to avoid relegation will be a tough one.
He added: "We're under no illusions. We've got a very tough run-in, we're right in the mix at the bottom, and there is still the possibility we could get relegated.
"But we've put ourselves in that position, and we're the only people who can get ourselves out of that position.
"The Liverpool win has to give us massive belief. We are not talking about an average side here. We played against a team packed with quality."