The Italian was seen to be calling for Maynor Figueroa to be sent off for a handball, but the defender was not sent off, with England striker questioning the gesture
Edin Dzeko scored the only goal of the game to put the blue half of Manchester three points clear of their title rivals, but in the dying moments of the game Maynor Figueroa handled the ball when acting as the last man, but referee Martin Atkinson did not believe the action deserved a sending off.
After seeing the handball, Mancini made a similar gesture to Rooney did when Vincent Kompany was sent off for a foul on Nani in the FA Cup third round game, while the Italian manager also appeared to be demanding the sending off of Martin Skrtel during his side's 3-0 victory over Liverpool.
Rooney wrote on Twitter during the game: "Was manchini (sic) asking for a red card????"
Mancini defended his actions after the final whistle, insisting it is acceptable for an incident such as the defender’s handball.
"I did it because Wayne Rooney did it," Mancini told reporters after the game. "It's normal with a chance like that to do it.
"I am on the bench. When you are near to the referee you can have more of an influence.
"I am on the bench and the referee cannot see me. I used to do this [as a player], but I don't want to say anything about that situation."