Chelsea have had two bids rejected for Rooney, the latest worth around £30 million, with the player desperate to complete a summer transfer to Stamford Bridge.
Rooney is set to feature for England in their international friendly against Scotland on Wednesday, having trained with the United reserves last week as he recovers from a shoulder injury, and Moyes is adamant that his position is unchanged.
"He's not for sale," the United boss told reporters at Wembley. "I'm not changing so there's no more to answer to your question.
|5/6||Wayne Rooney is 5/6 with Bet365 to be a Chelsea player on September 3
Moyes also moved to clarify his suggestion before the Community Shield that Rooney would be fit to play for England but not United’s Premier League opener against Swansea City on Saturday.
"I want him to join up with England," the Scot added. "He's not ready, you'll probably see that in the game. He's had no football, no match action. So you probably wouldn't expect someone in that condition to play for a long period.
"I've spoken to Roy Hodgson for two hours on different occasions. It will be great for Manchester United if he gets some playing time."
Moyes was speaking after winning the Community Shield with a 2-0 win over Wigan in his first official match as United manager - but the Scot was quick to give the credit to predecessor Sir Alex Ferguson.
"I class that as another piece of silverware for Sir Alex," said the new man graciously. "It was his team that won the Premier League last year. A big thank you to him, it's for him today. The next ones will be down to me."