Paul Cook's side loss 1-0 to Mansfield last time out but hold a four-point advantage over their promotion rivals, while Oxford host Southend and Fleetwood visit Bristol Rovers
Paul Cook's side were defeated 1-0 at home by neighbours Mansfield Town last weekend, but still remain four points clear of a group of three teams at the top of the table.
Chesterfield will look to extend or at least maintain that lead when they travel to ninth-place Morecambe on Saturday.
Mansfield, who have won three in a row, host third from bottom Hartlepool United, with just one win all season.
Second-place Oxford United, the division's top scorers and ahead of Mansfield on goal difference, play host to mid-table Southend United while Fleetwood Town in fourth visit Bristol Rovers.
Rochdale will look to extend their four-game winning streak when they host Portsmouth, while Exeter City, currently in sixth, are at home to Plymouth Argyle.
AFC Wimbledon occupy the final play-off spot and will be confident of continuing their strong start to the season when they host Northampton Town, who are without a win in seven matches.
Bottom club Accrington Stanley could pull level with Northampton on five points if they claim their first win of the campaign in Friday's home fixture with Dagenham and Redbridge.
Two struggling sides meet at Plainmoor as Torquay United, currently 18th, play host to Nigel Worthington's York City, a point and three places behind them in 21st.
Newport County have impressed after winning promotion from the Conference Premier last season and they will travel to Bury looking to muscle in on the play-off picture.
Elsewhere, Scunthorpe United, unbeaten in five matches, host Cheltenham Town and Wycombe Wanderers are at home to out-of-form Burton Albion, who have taken just one point from their previous four games.