The Catalans will host Alaves on May 27 as the curtain comes down on 50 years at Atletico Madrid's home stadium ahead of their move for 2017-18
This season's Copa del Rey final will be played at Atletico Madrid's Estadio Vicente Calderon, making it the last competitive match to be hosted at the stadium.
Barcelona beat Atletico over two legs in the semi-finals to earn a berth in the end-of-season showpiece and will take on Deportivo Alaves, who saw off Celta Vigo.
Atleti will now host the match as a result, with Real Madrid refusing to make the Santiago Bernabeu available for their rivals due to planned construction work.
Barca and Alaves will meet on Saturday, May 27, the weekend after Atletico's farewell to their current home against Athletic Club in La Liga.
The Catalans are looking to defend the trophy they have won in each of the past two seasons, defeating Sevilla at the Calderon last year to follow up a victory over Athletic at Camp Nou in 2015.
They also defeated the Basque club on Atletico's turf at the end of the 2011-12 season.
Since hosting the Copa final for the first time in 1973 following its opening six years earlier, the Calderon has been awarded the match 13 times.
Next season Atletico will move to the Wanda Metropolitano, a new 73,000-seater stadium on the site designated for Madrid's failed bid to host the Olympic Games in 2016.
The Calderon is set to be demolished once the season has ended and replaced with a waterfront park on the banks of the Manzanares river, where it sits.