Vicente del Bosque's side continue to dominate the proceedings as they ended up ahead of Germany and Argentina this year, while Colombia received the "Mover of the Year" titleSpain have finished 2012 at the summit of the Fifa Ranking for the fifth time in succession.
Joachim Low's Germany remain the second-placed team in the standings, with South American giants Argentina completing the podium.
Euro 2012 finalists Italy have climbed one place and now sit fourth in the ranking, ahead of Colombia who were named "Mover of the Year" after reaching fifth position from 36th at the start of the year.
England are sixth, while Portugal (seventh, three down), Netherlands (eighth, one down),Russia (ninth) and Croatia (10th) complete the rest of the top 10.
Copa America holders Uruguay have to settle for 16th place after dropping five places, while 2014 World Cup hosts Brazil are only 18th.
Cote d'Ivoire remain Africa's highest placed team at No.14, while Japan are the pride of Asia in 22nd position.
Cuba are this month's biggest climbers as they went 41 places up to make it to 100th spot, while Belize (down 23) dropped the most positions compared to last month's Fifa Ranking.