Antonio Conte will expect a response from his players as they seek to bounce back from their midweek Europa League defeat at Benfica in Monday's Serie A encounter
Eusebio Di Francesco's side have moved out of the drop-zone due to an impressive resurgence in the last three games, beating Atalanta and Chievo and earning a draw with Cagliari.
They face a stern test in attempting to continue their good run of form against Juventus, who head into the game on the back of a disappointing result in Europe in midweek.
The Turin giants slipped to a 2-1 defeat at Portuguese champions Benfica in the Europa League semi-final first leg on Thursday so will have to come from behind to reach the final at their own Juventus Stadium in May.
Coach Antonio Conte will expect a response from his side on Monday as they close in on another Scudetto.
Roma cut Juve's lead at the summit to five points with a 2-0 victory against AC Milan on Friday and the capital club will be hoping that Sassuolo do them a favour by securing a shock triumph.
However, the prospects of Di Francesco's men consigning the champions to only their third league loss of the campaign would appear slim, especially considering the heavy defeat that Conte's side inflicted on them in the reverse fixture.
Juve were comprehensive 4-0 winners on that occasion back in December, with Carlos Tevez scoring a hat-trick.
Sassuolo will face a near-fully fit Juve squad at the Stadio Citta del Tricolore, with midfielder Arturo Vidal their only absentee because of a knee injury which reportedly could require surgery.
Meanwhile, the hosts will be without three key players due to suspension: top scorer Domenico Berardi is banned, while midfielders Davide Biondini and Simone Missiroli are also suspended.