The Tigers defeated QPR in their opening Premier League fixture and the former Manchester United defender is keen to utilise the full depth of his squad on Thursday
The Tigers left it late to book their place in the play-offs of Europe's second-tier competition in their first ever continental campaign, an 80th-minute Sone Aluko goal required to give them a 2-1 aggregate win over Trencin of Slovakia in the third qualifying round, and they now meet a Belgian side who have won three of their last four matches.
The Yorkshire club opened their Premier League season with a 1-0 win at QPR on Saturday but Bruce had to leave out several of his seasoned campaigners and the former Birmingham City and Wigan manager is keen to use his strength in depth in Europe.
Bruce told Hull's official website: "Lokeren are a very, very good side, from what I've seen of them, and, as we know, Belgium is producing some very good players. It will be a difficult game for us.
"As I've said, I'll be changing my team around and giving the players who got us into Europe, the chance to play in Europe. I think working it that way is what gives us the spirit that we're playing with. They're all going to get their chance.
"I had to leave people off the bench at QPR on Saturday. George Boyd, in particular, who [has] done wonders for me."
Lokeren sit sixth in the Belgian top flight and will be buoyed by a 2-0 win over Lierse on Saturday. Their best result in European competition was a quarter-final appearance in the 1980-81 Uefa Cup.
Robert Snodgrass (knee) will miss out for Hull while defenders Alex Bruce and Liam Rosenior are doubtful with back injuries.