The Borussia Dortmund forward is suffering from gastroenteritis and will miss Germany's World Cup qualifier against Faroe Islands on Tuesday.
The 24-year-old forward started Friday's 3-0 win over Austria, assisting Toni Kroos' goal at the Allianz Arena, before making way for Julian Draxler before the finish.
However, he has since been diagnosed with a bout of gastroenteritis and will return to Dortmund for treatment.
A statement from the German FA read: "Marco Reus will not fly with the German national team to the Faroe Islands.
"The 24-year-old is suffering from a gastrointestinal infection."
Germany remains unbeaten in Group C having won six and drawn one of its seven matches, and can qualify for Brazil 2014 with a win in Torshavn, if Sweden fails to beat Kazakhstan in Astana.
In contrast, Lars Olsen's Faroe Islands side has lost all seven of its qualifiers.