The Fiorentina forward has been out of action for long spells this season but is included in the Azzurri's 30-man provisional set-up, alongside the Parma star.
The Azzurri have been drawn in Group D alongside Uruguay, England and Costa Rica and kick the tournament off with a game against Roy Hodgson's men on June 14.
Prandelli has included Fiorentina frontman Rossi, despite the former Villarreal star playing in only two Serie A matches since early January having just recovered from a cruciate ligament injury.
Cassano also makes the cut after some excellent performances for Parma this term, while Torino star and Serie A top goalscorer Immobile has also been included alongside Mattia Destro, Lorenzo Insigne and Marco Verratti. Hellas' Brazilian-born midfielder Romulo is also on the list.
Usual suspects Daniele De Rossi, Andrea Pirlo, Mario Balotelli, Gianluigi Buffon, Claudio Marchisio, Thiago Motta and Giorgio Chiellini are present in the squad, but there is no place for AC Milan's Stephan El Shaarawy, who only returned to action last week after months on the sidelines through injury.
Squad in full:
Goalkeepers: Gianluigi Buffon (Juventus), Mattia Perin (Genoa), Salvatore Sirigu (Paris Saint-Germain), Antonio Mirante (Parma)
Defenders: Ignazio Abate (AC Milan), Andrea Barzagli (Juventus), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Matteo Darmian (Torino), Mattia De Sciglio (AC Milan), Christian Maggio (Napoli), Gabriel Paletta (Parma), Manuel Pasqual (Fiorentina), Andrea Ranocchia (Inter)
Midfielders: Alberto Aquilani (Fiorentina), Antonio Candreva (Lazio), Daniele De Rossi (Roma), Claudio Marchisio (Juventus), Riccardo Montolivo (AC Milan), Thiago Motta (Paris Saint-Germain), Marco Parolo (Parma), Andrea Pirlo (Juventus), Romulo (Verona), Marco Verratti (Paris Saint-Germain)
Forwards: Mario Balotelli (AC Milan), Antonio Cassano (Parma), Alessio Cerci (Torino), Mattia Destro (Roma), Ciro Immobile (Torino), Lorenzo Insigne (Napoli), Giuseppe Rossi (Fiorentina).