Steve Kean's men have dropped to the Championship for the first time since 1999 after failing to beat Lancashire rivals Wigan at Ewood Park on Monday night
The Lancashire outfit fall out of the top flight for the first time since the 1998-99 campaign in which it finished 19th - the position it will occupy this term.
Steve Kean's men have been battling at the bottom of the table for much of the season and required two wins from their last two games to have any chance of survival.
Although results went their way at the weekend, Rovers failed to take advantage at Ewood Park, with Antolin Alcaraz's later header consigning them to the Championship.
Rovers will be relegated alongside Wolves, whose fate was sealed after their 2-0 defeat to Manchester City in April, and will be joined by either QPR or Bolton on the final day of the season.
Reading and Southampton are set to replace them after gaining automatic promotion, and they will be joined by the winners of the Championship playoff final.