The club formerly known as Melbourne Heart host the Sky Blues in Ballarat, where John van 't Schip's men are $2 favourites at bet365.
Graham Arnold's visitors are paying a generous $3.25 to reach the last 16, with the draw worth $3.40.
Elsewhere on Tuesday, former National Soccer League side Adelaide City are a lengthy $5 to get the better of last season's beaten grand finalists.
Tony Popovic's men will surely have one eye on their forthcoming AFC Champions League quarter-final with Chinese Super League powerhouse Guangzhou Evergrande.
Nevertheless, punters are backing the Wanderers to get the job done and they are offering a return of just $1.50 to win in South Australia this week.
In the other games, Blacktown City ($1.90) are expected to get the better of Bentleigh Greens ($3.10), while Sydney United 58 ($2) against FNQ FC Heat ($2.87) is tougher to call.