The 17-year-old spent six days in the intensive care unit after being knocked from his scooter last week but his condition never improved and he passed away on Thursday
Mirko Fersini was left in a coma after suffering serious head injuries in a collision with a metal bin while riding on his moped in Fiumicino on April 6.
The 17-year-old defender, who was a member of Simone Inzaghi's Allievi Nazionali team, spent the past six days in intensive care but his condition never improved and on Thursday he passed away.
"After the tragic incident in which he was involved, the heart of Mirko Fersini has stopped beating this afternoon," a statement on the Lazio website read.
"The family of the young Biancocelesti defender has given permission to donate the organs.
"Lazio president Claudio Lotito, the coach [Edy Reja], players and all the staff will gather around the family of Mirko in this moment of extreme grief.
"As a sign of mourning, the club has lowered the flags to half-mast at Formello."
Biancocelesti fans had displayed banners of support for Fersini during their Serie A clash with Napoli last Saturday.