FC Twente Enschede 
| 1 Sander Boschker | T | 23 Nashat Akram | M | |
| 13 Nikolay Mihaylov | T | 24 Theo Janssen | M | |
| 16 Cees Paauwe | T | 25 Andrej Rendla | M | |
| - Dwight Tiendalli | V | 33 Alexander Bannink | M | |
| 2 David Carney | V | - Ransford Osei | S | |
| 3 Nicky Kuiper | V | - Wellington | S | |
| 4 Peter Wisgerhof | V | 9 Blaise N'Kufo | S | |
| 5 Slobodan Rajkovic | V | 11 Nikita Rukavytsya | S | |
| 8 Ronnie Stam | V | 14 Bernard Parker | S | |
| 12 Jeroen Heubach | V | 15 Miroslav Stoch | S | |
| 19 Douglas | V | 20 Luuk de Jong | S | |
| - Perparim Hetemaj | M | 22 Bryan Ruiz | S | |
| 6 Wout Brama | M | 27 Dario Vujicevic | S | |
| 10 Kenneth Perez | M | - Steve McClaren | Tr | |
| 18 Cheikh Ismael Tiote | M |
| Spieler | Tore | Elfmeter | ||
|---|---|---|---|---|
 | Bryan Ruiz FC Twente Enschede | S | 12 | 0 |
 | B. N'Kufo FC Twente Enschede | role | 11 | 2 |
 | M. Stoch FC Twente Enschede | M | 6 | 0 |
 | Douglas FC Twente Enschede | V | 5 | 1 |
 | R. Denneboom FC Twente Enschede | S | 3 | 0 |
| Spieler wechselt |
zu |
von |
|---|
| Willem II Tilburg - FC Twente Enschede | 28.11.09 |
| FC Twente Enschede - Fenerbahce | 02.12.09 |
| NEC Nijmegen - FC Twente Enschede | 06.12.09 |
| FC Twente Enschede - NAC Breda | 12.12.09 |
| Steaua - FC Twente Enschede | 17.12.09 |
| FC Utrecht - FC Twente Enschede | 20.12.09 |
| Groningen - FC Twente Enschede | 24.01.10 |
| FC Twente Enschede - Roda JC Kerkrade | 31.01.10 |
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